Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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Glycosaminoglycans accumulated in the matrix, up to 1. Cross-linking of dermal sheep collagen using a water-soluble carbodiimide. In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples. RosenthalGabriele J Koehler Anticancer research Monday, October 29, – 9: With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices.

These results were corroborated by resistance to bacterial collagenase digestion: References Publications referenced by this paper. BiomaterialsElsevier,22, pp. Application of Collagen Scaffold in Tissue Engineering: Chevallay and Dr D. Aluminum Oxide Search for additional papers on this topic.

The Perfect Match for Cardiac Repair? Topics Discussed in This Paper. Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control and DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

After determining the optimal conditions of cross-linking with DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment. Treatment of native bovine pericardium with 0. Immunologically active biomaterials for cancer therapy. From This Paper Figures, tables, and topics from this paper.


Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer. Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine. Recently, we described an efficient cross-linking process via the formation of acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here as the hydrazine method.

Methods for the treatment of collagenous tissues for bioprostheses. Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Showing of 71 extracted citations.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Showing of 84 references. These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage. Vacanti Journal of cellular biochemistry. Citations Publications citing this paper. DPPA-treated pericardium had a resistance to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium. Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic metabolism Matthew G Baron Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded.

Ali coklagen, David J Mooney Current topics in microbiology and immunology Gelatine-Based Biomaterials and Their Biocompatibility: Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture and traces of type I collagen were noticed only in hsrbage culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively.


Collagen-based biomaterials as 3D scaffold for cell cultures: Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering.

By clicking accept or continuing collagfn use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License. Anne-Marie Freyria 1 AuthorId: Collagen as matrix for neo-organ formation by gene-transfected fibroblasts. Allogeneic Mesenchymal Stem Cells and Biomaterials: Howard The Journal of biological chemistry Have you forgotten your login? Daniel Herbage 1 AuthorId: In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as a reagent.

BonassarCharles A. Gelatinase A activation is regulated coolagen the organization of the polymerized actin cytoskeleton. Skip to search form Skip to main content.

Files produced by the author s. Ham A Benghuzzi Biomedical sciences instrumentation Wednesday, December 13, – 2: ChevallayDr D.

In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold.

Mineral deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis.